Genotoxic potential of aqueous extract fresh sheets do grass - lemon (Cymbopogon citratus (A.D.) Stapf). In mice in micronucleus test means

Authors

  • Juscélio Clemente de Abreu University Center of Caratinga
  • Lamara Laguardia Valente Rocha University Center of Caratinga
  • Eugënio Maria Gomes University Center of Caratinga
  • Wanessa Soares Luiz Silva University Center of Caratinga
  • Leonardo Ricardo Soares College Vale do Cricaré
  • Natanael Átila Aleva College PROMOVE of Technology of Belo Horizonte
  • Daniel Rodrigues Silva University Center of Caratinga

DOI:

https://doi.org/10.31686/ijier.vol7.iss6.1521

Keywords:

Cymbopogoncitratus(A.D.) Stapf, Genotoxicity, micronucleus

Abstract

Lemongrass (Cymbopogon citratus) it is a medicinal herb grown in almost all tropical countries, including Brazil. Your still therapeutic use is homemade, taking into account the ethnobotanical knowledge. Despite the long history with no reports of serious side effects, research has shown that they may have genotoxic potentialities. Therefore, this study aimed to examine the genotoxic effects of aqueous extract of lemongrass, prepared in 2%, 4% and 8% in the animal test system - Mus musculus L. The results showed that the bend the concentration of the aqueous extract of fresh leaves of lemon grass, bent also genotoxic effects on bone marrow Mus musculus L, inducing an increased frequency of micronucleus. This way, the consumption of lemon grass tea should be done with moderations and monitoring of health professionals.

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Author Biography

  • Leonardo Ricardo Soares, College Vale do Cricaré

    Master Science Technology and Education

References

ABREU, J.C. at et. Effects cytotoxic and genotoxic of aqueous extract of fennel (Foeniculum vulgare var. vulgare Mill.), International Journal of Advanced Engineering Research and Science, v. 6, n. 3, p. 230-236, 2019.AKINBORO, A., BAKARE, A.A. Cytotoxic and genotoxic effects of aqueous extracts of five medicinal plants on Allium cepa Linn. J. Ethnopharmacol, 112, p. 470–475, 2007. DOI: https://doi.org/10.1016/j.jep.2007.04.014

AZEEZ, et al. Proximate and phytochemical constituents of four medicinal plants and their cytogenotoxic effects using Allium cepa assay, Journal of Agroalimentary Processes and Technologies, v. 22, n. 3, p. 132-141, 2016.

BIDINOTTO, L.T.; COSTA, C.T.; SALVADORI, D.M. et al. Protective effects of lemon grass (Cymbopogon citrates Stapf) essential oil on DNA damageandcarcinogenesis in female Balb/C mice. J Appl Toxicol, v. 31, n. 6, p. 536–554, 2011. DOI: https://doi.org/10.1002/jat.1593

BRASIL. Ministério da Saúde. Secretaria de Ciência, Tecnologia e InsumosEstratégicos. Departamento de AssistênciaFarmacêutica. A fitoterapia no SUS e o Programa de Pesquisa de PlantasMedicinais da Central de Medicamentos. Brasília: Ministério da Saúde, 2006. 148 p. – (Série B. TextosBásicos de Saúde).

COSTA, C.A.R.A. et al. Cholesterol reduction and lack of genotoxic ortoxic effects in mice afterrepeat ed 21-day oral in take of lemon grass (Cymbopogon citratus) essential oil. Food and Chemical Toxicology, v. 49, p. 2268–2272, 2011. DOI: https://doi.org/10.1016/j.fct.2011.06.025

CROWELL, P.L.; ELSON, C.E.; BAILEY, H.H. et al. Human metabolism of the experimental cancer therapeutic agent d-limonene. Cancer Chemother Pharmacol, v. 35, p. 31–37, 1994. DOI: https://doi.org/10.1007/BF00686281

FAGUNDES, et al. Avaliação da citotoxicidade de trêsplantasmedicinaisencontradasemquintaisurbanos no município de Alta Floresta, Mato Grosso, Brasil, EnciclopédiaBiosfera, v.14, n.26, p. 806-815, 2017. DOI: https://doi.org/10.18677/EnciBio_2017B70

FANDOHAN, P.; GNONLONFIN, B.; LALEYE, A. et al. Toxicity and gastric tolerance of essential oils from Cymbopogon citratus, Ocimumgratissimum and Ocimumbasilicum in Wistar rats. Food ChemToxicol, v. 46, 2493–2497, 2008. DOI: https://doi.org/10.1016/j.fct.2008.04.006

FURLAN, M.R. et al. Variação dos teores de constituintesvoláteis de Cymbopogon citratus (DC) Staf, Poaceae, coletadosemdiferentesregiões do Estado de São Paulo. Rev. bras. farmacogn., v. 20, n. 5, p. 686-691, 2010. DOI: https://doi.org/10.1590/S0102-695X2010005000026

GUERRA, M.J.M. et al. Toxicologic acute evaluation of the fluid extracts 30 and 80 porciento of Cymbopogon citratus(D.C.) Stapf (lemongrass). Rev Cub Plantas Med, v. 5, 97–101, 2000.

GUIMARÃES, L.G.L. et al. Influência da luz e da temperaturasobreaoxidação do óleoessencial de capimlimão (Cymbopogon citratus (D.C.) Stapf). Química Nova, v. 31, n. 6, p. 1476-1480, 2008.HANAA, A.R. et al. Lemon grass (Cymbopogoncitratus) essential oil as affectedbydryingmethods. Annals of Agricultural Science, v. 57 n. 2, p. 113– 116, 2012. DOI: https://doi.org/10.1590/S0100-40422008000600037

KUMAR, A. et al. Assessment of Thymus vulgaris L. essential oil as a safe botanical preservative against post harvest fungal infestation of food commodities. Innovative Food Science andEmerging Technologies, v. 9, p. 575-580, 2008. DOI: https://doi.org/10.1016/j.ifset.2007.12.005

LAGARTO, P.A; SILVA, Y.R.; GUERRA, S.I. et al. Comparative study of the assay ofArtemiasalina L. andtheestimateofthemediumlethal dose (LD50 value) in mice, to determine oral acutetoxicityofplantextracts. Phytomedicine, v.8, n. 5, p. 395–400, 2001. DOI: https://doi.org/10.1078/0944-7113-00044

LEMOS, G.C.S. et al. Controle de plantasinvasorasemcultivoorgânico e convencional de capim-limão (Cymbopogoncitratus). RevistaBrasileira Pl. Med., v. 15, n. 3, p. 405-414, 2013. DOI: https://doi.org/10.1590/S1516-05722013000300014

LUCENA BFF, TINTINO SR, FIGUEREDO FG, OLIVEIRA CDM, AGUIAR JJS, CARDOSO EN, AQUINO PEA, ANDRADE JC, COUTINHO HDM, MATIAS

EFF. Avaliação da atividadeantibacteriana e moduladora de aminoglicosídeos do óleoessencial de Cymbopogoncitratus(DC.) Stapf. Acta biol. Colomb, v. 20, n. 1, p. 39-45, 2015. DOI: https://doi.org/10.15446/abc.v20n1.41673

MACHRAOUI, M.;KTHIRI, Z.; BEN JABEUR, M.; HAMADA, W. Ethnobotanicalandphytopharmacological notes onCymbopogoncitratus (DC.) Stapf, Journalof new sciences, AgricultureandBiotechnology, v. 55, n. 5, p. 3642-3652, 2018.

MARTINAZZO, A.P.; CORREA, P.C.; MELO, E.C.; BARBOSA, F.F. Difusidadeefetivaemfolhas de Cymbopogoncitratus (D.C) Stapfsubmetidas à secagem com diferentescomprimentos de corte e temperaturas do ar. RevistaBrasileira de PlantasMedicinais, v.9, n.1, p.68-72, 2007.

MEEVATEE, U.; BOONTIM, S.; KEEREETA, O.; VINITKETKUMNUEM, U.; OARIYAKUL, N. Antimutagenicactivityoflemongrass. In: BOONTIM, S. editor. Man andEnvironment. Chiang Mai: Chiang Mai University Press; 1993. p. 346.

NEGRELLE, R.R.B.; GOMES, E.C. Cymbopogoncitratus(D.C) Stapf: chemicalcompositionandbiologicalactivities. RevBrasPl Med. v. 9, p. 80–92, 2007.

NOGUEIRA, A.C.M.A.; CARVALHO, R.R.; SOUZA, C.A.M. et al.

Studyontheembryofoeto-toxicityofcitral in therat. Toxicology, v. 196, p. 105– 113, 1995.

PAREDES, S.; GAYOZO, E. Actividadalelopáticadelextractoetanólico deCymbopogonnardus L. sobregerminación y crecimiento radicular de

Phaseolusvulgaris L. Steviana, v. 10, n. 2, p. 17 – 23, 2018. DOI: https://doi.org/10.56152/StevianaFacenV10N2_2018

PEREIRA, P.S.; PAULA, L.L.R.J. “Açõesterapêuticas do capim-santo: umarevisão de literatura”. RevistaSaúdeemFoco, n. 10, 2018.

PINTO, D.A. et al. Produtividade e qualidade do óleoessencial de capim-limão, Cymbopogoncitratus, DC., submetido a diferenteslâminas de irrigação. Rev. bras. plantas med., Botucatu, v. 16, n. 1, p. 54-61, 2014. DOI: https://doi.org/10.1590/S1516-05722014000100008

PUATANACHOKCHAI, R.; KISHIDA, H.; DENDA, A.; MURATA, N.; KONISHI,

Y.; VINITKETKUMNUEN, U. et al. Inhibitoryeffectsoflemongrass

(Cymbopogoncitratus, Stapf)

extractontheearlyphaseofhepatocarcinogenesisafterinitiationwithdiethylnitrosami ne in male Fischer 344 rats. CancerLett., v. 183, n. 1, p. 9-15, 2002. DOI: https://doi.org/10.1016/S0304-3835(02)00111-8

RAUBER, C.S.; GUTERRES, S.S.; SCHAPOVAL, E.E.S. LC determinationofcitral in Cymbopogoncitratusvolatileoil. J. Pharma. Biomed. Anal. 37, 597-601, 2005. DOI: https://doi.org/10.1016/j.jpba.2004.10.042

SACCHETTI, G.; MAIETTI, S.; MUZZOLI, M.; SCAGLIANTI, M.; MANFREDINI, S.; RADICE, M.; BRUNI, R. Comparativeevaluationof 11 essentialoilsofdifferentorigin as functionalantioxidants, antiradicalsandantimicrobials in foods. Food Chemistry, v. 91, n. 4, p. 621-632, 2005. DOI: https://doi.org/10.1016/j.foodchem.2004.06.031

SANTIN, M.R.; SANTOS, A.O.; NAKAMURA, C.V. et al. In vitro activityoftheessentialoilofCymbopogoncitratusand its major component (citral) on Leishmania amazonensis. Parasitol Res, v. 105, p. 1489–1496, 2009. DOI: https://doi.org/10.1007/s00436-009-1578-7

SANTOS NETO, I.R.; ALVES, M.G.L.; MARTINS, M.T.C.S. Utilização de plantasmedicinaispelosgrupos de idosos e de jovens no município de Parari – PB. RevistaAcadêmicocientífica, v. 7, n. 1, p. 1-15, 2015.

SANTOS, A. et al. Determinação do rendimento e atividadeantimicrobiana do óleoessencial de Cymbopogoncitratus(DC.) Stapfemfunção de sazonalidade e consorciamento. RevBrasFarmacogn, v. 19, n. 2, p. 436-441, 2009. DOI: https://doi.org/10.1590/S0102-695X2009000300017

SILVA, L.P.; RECK, R.T.; FONSECA, F.N. Desenvolvimento de formasfarmacêuticassemissólidas a partir de capim-limão (Cymbopogoncitratus). SaúdeMeio Ambient. v. 5, n. 2, p. 82-92, 2016.SIMÕES, C.M.O.; SCHENKEL, E.P.; GOSMANN, G.; de MELLO, J.C.P.; MENTZ, L.A.; PETROVICK, P.R. Farmacognosia: da planta aomedicamento. 4.ed., Porto Alegre/Florianópolis: Ed. Universidade/ UFRGS/Ed.UFSC, 2002. 833p. DOI: https://doi.org/10.24302/sma.v5i2.1069

SOUSA, S.M.; SILVA, P.S.; VICCINI, L.F. Citogenotoxicidade de extratosaquosos de Cymbopogoncitratus (DC) Stapf (capim-limão) emsistemas de testes vegetais. A. Acad. Bras. Ciênc. Rio de Janeiro, v. 82, n. 2, p. 305-311, 2010. DOI: https://doi.org/10.1590/S0001-37652010000200006

SOUZA, A.C.S. et al. Citralpresentescytotoxicandgenotoxiceffects in humanculturedcells, DrugandChemicalToxicol., 2019.

DOI:10.1080/01480545.2019.1585445 DOI: https://doi.org/10.1080/01480545.2019.1585445

SOUZA, S.A.M. et al. Utilização de sementes de alface e de rúculacomoensaiosbiológicos para avaliação do efeitocitotóxico e alelopático de extratosaquosos de plantasmedicinais, Revista de Biologia e Ciências da Terra, v. 5, n. 1, 2005.

SUAEYUN, R.; KINOUCHI, T.; ARIMOCHI, H.; VINITKETKUMNUEM, U.; OHNISHI, Y. Inhibitoryeffectsoflemongrass (Cymbopogoncitratus, Stapf) onformationofazoxymethane-induced DNA adductsandaberrantcryptfoci in theratcolon. Carcinogenesis, v. 18, n. 5, p. 949-955, 1997 DOI: https://doi.org/10.1093/carcin/18.5.949

VIGUSHIN, D.M.; POON, G.K.; BODDY, A. et al. Phase I andpharmacokineticstudyof d-limonene in patientswithadvancedcancer. Cancer Research Campaign Phase I/II Clinical Trials Committee. Cancer Chemother Pharmacol, v. 42, p. 111–117, 1998. DOI: https://doi.org/10.1007/s002800050793

VILLAVERDE, J.M., et al. Effects of essential oil from lemon grass (Cymbopogon citratusStapf) on human melanoma (SK-MEL 147) and keratinocyte (HaCaT) cells. Biosaúde, v. 15, n. 1, p. 22-36.

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Published

2019-06-01

How to Cite

Abreu, J. C. de ., Rocha, L. L. V. ., Gomes, E. M. ., Silva, W. S. L. ., Soares, L. R. ., Aleva , N. Átila ., & Silva, D. R. . (2019). Genotoxic potential of aqueous extract fresh sheets do grass - lemon (Cymbopogon citratus (A.D.) Stapf). In mice in micronucleus test means. International Journal for Innovation Education and Research, 7(6), 7-17. https://doi.org/10.31686/ijier.vol7.iss6.1521

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