Solid state fermentation of soybean meal with aspergillus Niger for upgrading nutritive values

Authors

  • Hsu Thet Hmue Naing University of Veterinary Science, Myanmar
  • Khin Hnin Swe University of Veterinary Science, Myanmar
  • Khin San Muu University of Veterinary Science, Myanmar

DOI:

https://doi.org/10.31686/ijier.vol7.iss5.1531

Keywords:

Aspergillus niger, Nutritional value, Phytase activity, Soybean meal, Solid state fermentation

Abstract

The experiment was conducted to observe the effect of different fermentation times on nutrient compositions and phytase activity of fermented soybean meal (FSBM) used solid state fermentation (SSF) by Aspergillus niger. A completely randomized design (CRD) was used in this experiment which was included three treatments and replicated three times per treatment. Soybean meal was used as substrate and inoculated with A. niger spore suspension. Two hundred and fifty ml capacity of Erlenmeyer flask was used to cultivate the culture. The fermentation times were 0, 3 and 5 days. The contents of dry matter, ash, ether extract, crude protein, gross energy and phytase activity of fermented soybean meal were determined. The percentages of crude protein, gross energy and phytase activity of day 5 FSBM were significantly (p<0.05) higher than those of day 3 FSBM. Fermented SBM at day 5 had numerically increased in the ether extract contents. However, the ash and dry matter percentages of day 5 FSBM were significantly (p<0.05) reduced compared with those of day 3 FSBM. According to the obtained results, the effect of different fermentation times on nutrient contents and phytase activity of fermented soybean meal were significantly different. Therefore, fermented SBM incubated at day 5 is an appropriate alternative feed from SBM to be used as nourishable feed and phytase enzyme production in fermented soybean meal which will being economic benefits to farmers.

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Author Biography

  • Hsu Thet Hmue Naing, University of Veterinary Science, Myanmar

    Master of Animal Nutrition

References

AOAC(1995). Association of Official Analytical Chemist. Official methods of analysis,16th edn. Gaithersburgh, MD. USA, Vol. II :24-32.
Ashok P, Carlos RS, Jose AR, Poonam N (2003).Solid State Fermentation. Biochemical engineering journal, 13:3-5.
Ashoke A, Doriya K, Rao DRM, Kumar DS (2017).Design of solid state bioreactor for industrial applications : An
Bimrew A (2014).Biotechnological Advances for Animal Nutrition and Feed Improvement. World Journal of Agricultural Research,2 (3): 115-118.
Cromwell, GL, Stahly TS, Coffey RD, Monegue HJ and Randolph JH (1993). Efficiency of phytase in improving the bioavailability of phosphorus in soybean meal and corn-soybean meal diets for pigs. J. Anim. Sci. 71:1831-1840.
Ebune A, Al-Ashel S and Duvnjak Z (1995). Production of phytase during solid state fermentation using Aspergillus ficuum NRRL 3135 in canola meal. Bioresource Technology, 53(1): 7-12.
Feddern V, Souza- Soares LA, Badiale- Furlong, E (2007) Efeitos da fermentaçăo nas propriedades fîsicoquímicas e nutricionais do farelo de arroz. Ciênc. Technol.Aliment.27: 800-804.
Frias J, Song YS, Martinez-Villaluenga C, Gonza’lez de ME, Vidal-Valverde C (2007). Immunoreactivity and amino acid content of fermented soybean products. J.Agri Food Chem, 59(1): 99-105.
Gautam P, Sabu A, Pandey A, Szakacs G, Soccol CR (2002). Microbial production of extra-cellular phytase using polystyrene as inert solid support. Bioresour Technol, 83(3): 229-233.
Hirabayashi M, Matsui T, Yano H and Nakajima T (1998). Fermentation of soybean meal with Aspergillus usamii reduces phosphorus excretion in chicks. Poult. Sci. 77: 552-556.
Hong KJ, Lee CH and Kim SW (2004). Aspergillus oryzae GB-107 Fermentation Improves Nutritional Quality of Food Soybeans and Feed Soybean Meals. Journal of Medical Food, 7(4): 430-435.
Kim SW, Heugten E, Ji F, Lee CH, Mateu RD (2010) Fermented soybean meal as a vegetable protein source for nursery pigs: I. Effects on growth performance of nursery pigs. Journal of Animal Science. 88: 214-224.
Kupski L, Cipolatti E, da Rocha M, Oliveira M da S, Souza-Soares L de A, Badiale-Furlong E (2012). Solid state fermentation for the enrichment and extraction of proteins and antioxidant compounds in rice bran by Rhizopus oryzae. Brazilian archives of biology and technology, 55(6): 937-942.
Larroche C and Gros JB (1997).Advances in biochemical engineering/biotechnology. J. Pubmed, 55:179-220.
Lawal TE, Ademola SG, Owoseni A, Atobatele OE and Oriye LO (2013). Use of Aspergillus niger for improving the feeding value of rice offal. African journal of biotechnology, 12(20): 3083- 3087.
Liener IE (1994).Implication of antinutritional components in soybean foods. Crit. Rev. Food Sci.Nutr. 34:31-67.
Liener IE (2000).Non-Nutritive Factors and Bioactive Compounds in Soy. Soy in Animal nutrition Symposium (August 45Chicago, IL), (Ed. J.K. Drackley) Fed. An. Sci. Societies, Savoy.
Mandviwala TN and Khire JM (2000). Production of high activity thermostable phytase from thermotolerant Aspergillus niger in solid state fermentation. Journal of Industrial Microbiology and Biotechnology, 24: 237- 243.
Oboh G, Akindahunsi AA, Oshodi AA (2002). Nutrient and anti-nutrient content of Aspergillus niger fermented cassava products (flour and gari). J Food Comp Anal, 15:617-622.
overview to conventional bioreactors. Biocatalysis and Agricultural Biotechnology, 9: 11-18.
Pal Vig A and Walia A (2001). Beneficial effects of Rhizopus oligosporus fermentation on reduction of glucosinolates, fiber and phytic acid in rapeseed (Brassica napus) meal. Bioresources Technology, 78: 309-312.
Ramachandran S, Krishnan R, Nampoothiri KM, Szakacs G, Pandey A (2005) Mixed substrate fermentation for the production of phytase by Rhizopus spp. using oil cakes as substrates. Proc. Biochem, 40 (5) :1749-1754.
Ramachandran S, Roopech K, Nampoothiri KM, Szakacs G, Pandey A (2005). Mixed substrate fermentation for the production of phytase by Rhizopus spp. using oilcakes as substrates. Process Biochem, 40(5): 1749-1754.
Rozan P, Villaume C, Bau HM, Schwertz A, Nicolas JP, Me´jean L (1996). Detoxification of rapeseed meal by Rhizopus oligosporus sp- T3: a first step towards rapeseed protein concentrate. International Journal of Food science and Technology. 31(1): 85-90.
Safari O, Farhangi M, Carter C, Yakhchali B, Bai S, Sangatash MM (2012). Study on the effect of solid state fermentation with Aspergillus niger on antinutritional factors of canola protein concentrate with aim of using in the diet of rainbow trout (Oncorhynchus mykiss). The 1st international and the 4th National Congress on Recycling of Organic waste in Agriculture, 26-27.
Saithi S and Tongta A (2016), Phytase production of Aspergillus niger on soybean meal by solid state fermentation using a rotating Drum Bioreactor, International Conference on Invention & Innovations for Sustainable Agriculture ICIISA.
Sara Willis (2003).The use of soybean meal and full fat soybean meal by the animal feed industry. Proceeding of 12th Australian Soybean Conference, Toowoomba, Qld, Australia.
SAS® Institute (2002). SSAT/ STAT User’s Guide Release Version 6.03ed. SAS® institute Inc., Cary, N.C.
Song YS, Pe’rez VG, Pettigrew JE, MartineZ-Villaluenga C and Mejia de EG (2010). Fermentation of soybean meal and its inclusion in diets for newly weaned pigs reduced diarrhea and measures of immunoreactivity in the plasma. Anim. Feed Sci. Technol. 159:41-49.
Soni SK and Khire JM (2007). World J of Microbiol, Biotechnol, 123: 1585.
Tadeusz P (2010). Cultivation of R zepak. Rape market-state and perspectives.
Teresa B (2011). Nutritional value of soybean meal.
Tian M and Yuan Q (2016). Optimization of phytase production from potato waste using Aspergillus ficuum. Journal of biotech, springer. 6: 256.
Wang ZH, Dong XF, Zhang GQ, Tong JM, Zhang Q, Xu SZ (2011).Waste vinegar residue as substrate for phytase production. Waste Manage Res, 29(12): 1262-1270.
Yamamoto M, Saleh F, Tahir M, Ohtsuka A, Hayashi K (2007). The effect of koji-feed (fermented distillery by-product) on the growth performance and nutrient metabolizability in broiler. Journal of Poultry Science. 44: 291-296.

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Published

2019-05-01

How to Cite

Naing, H. T. H., Swe, K. H. S., & Muu, K. S. M. (2019). Solid state fermentation of soybean meal with aspergillus Niger for upgrading nutritive values. International Journal for Innovation Education and Research, 7(5), 374-381. https://doi.org/10.31686/ijier.vol7.iss5.1531