Neurotoxicity evaluation of meloxicam in the alternative in vivo model, Caenorhabditis elegans

Juliana  Cyrillo Guimarães da Silva; Cassiana  Bigolin; Laura Cé da Silva; Thalia Emmanoella  Sebulsqui Saraiva; Julia  Machado Menezes; Andriele Veiverberg; Mariele  Feiffer Charão; Andresa Heemann Betti

doi:10.31686/ijier.vol8.iss8.2522

Authors

Juliana Cyrillo Guimarães da Silva Graduate Program in Toxicology and Experimental Toxicology, Institute of Health Sciences, Feevale University, Novo Hamburgo, Rio Grande do Sul, Brazil; Department of Bioanalysis, Institute of Health Sciences, Feevale University, Novo Hamburgo, Brazil.
Cassiana Bigolin Graduate Program in Toxicology and Experimental Toxicology, Institute of Health Sciences, Feevale University, Novo Hamburgo, Rio Grande do Sul, Brazil. Department of Bioanalysis, Institute of Health Sciences, Feevale University, Novo Hamburgo, Brazil.
Laura Cé da Silva Department of Bioanalysis, Institute of Health Sciences, Feevale University, Novo Hamburgo, Brazil.
Thalia Emmanoella Sebulsqui Saraiva Graduate Program in Toxicology and Experimental Toxicology, Institute of Health Sciences, Feevale University, Novo Hamburgo, Rio Grande do Sul, Brazil. Department of Bioanalysis, Institute of Health Sciences, Feevale University, Novo Hamburgo, Brazil.
Julia Machado Menezes Department of Bioanalysis, Institute of Health Sciences, Feevale University, Novo Hamburgo, Brazil.
Andriele Veiverberg Department of Bioanalysis, Institute of Health Sciences, Feevale University, Novo Hamburgo, Brazil.
Mariele Feiffer Charão Graduate Program in Toxicology and Experimental Toxicology, Institute of Health Sciences, Feevale University, Novo Hamburgo, Rio Grande do Sul, Brazil. Department of Bioanalysis, Institute of Health Sciences, Feevale University, Novo Hamburgo, Brazil.
Andresa Heemann Betti Graduate Program in Toxicology and Experimental Toxicology, Institute of Health Sciences, Feevale University, Novo Hamburgo, Rio Grande do Sul, Brazil. Department of Bioanalysis, Institute of Health Sciences, Feevale University, Novo Hamburgo, Brazil. https://orcid.org/0000-0002-7116-5465

DOI:

https://doi.org/10.31686/ijier.vol8.iss8.2522

Keywords:

C. elegans, neurotoxicity, meloxicam, NSAID

Abstract

Inflammatory processes cause changes in the permeability of the blood brain barrier. Non-steroidal anti-inflammatory drugs (NSAID) are most commonly used to treat these inflammatory processes, including meloxicam, and they can reach the central nervous system (CNS) and cause neurotoxicity. Since there are no studies evaluating the neurotoxicity of NSAID in alternative models of toxicity, the aim of this study was to evaluate the acute neurotoxicity (through nematodes changes in behavior) of meloxicam in an alternative in vivo model, Caenorhabditis elegans, as well as, to determine meloxicam toxicity through LD₅₀ and development assessments. Meloxicam LD₅₀ was high (50.03 mg/mL) and only the highest dose (100 mg/mL) caused a decrease in the nematode body size, indicating low toxicity in this alternative model. Besides, a neurological effect was observed only in the highest dose. Meloxicam showed neurotoxicity only at a very high dose, suggesting low potential to cause toxicity in the CNS. However, further studies are necessary to evaluate meloxicam neurotoxicity.

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