Improvement of Callogenesis Ability by Selecting a Better Hormonal Balance in Potato (Solanum tuberosum L).
DOI:
https://doi.org/10.31686/ijier.vol4.iss9.589Keywords:
Potato cultivars, callus culture, in vitro, hormonal balanceAbstract
Response in tissue culture is highly genotype dependent, significant genotypic diferences in callus initiation response were observed among both potato genotype investigated. The Spunta variety devleops the best calogenesis in all media compared to Kondor variety depending on hormones concentration,there is a range of variations in days needed for callus initiation,percentage of explant that developed callus, its texture, color and the degre of its formation.Our resutls chow that the callus depends on explant type.Sprout explant respond best to callus formation. the amount of callus ranges from 60% to 90 % for Spunta .Callus color after eight weekwas light green or green yelow for both varieties.The higest amount of callus 100% was obtained with the combination (NAAxBAP)(0.5/1, medium M2) and (2/2 ,medium M3) with Kondor bud explant .In media M1(1/0.5 mg/l),M2 (0.5/1 mg/l) and M3 (2/2 mg/l) with sprout explant of Spunta the amount of 80 %was noted,callus in media M1 and M2 produced microtubers,shoots and roots.
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References
Arora, A., & Chawla, H. S. (2005). Organogenic plant regeneration via callus induction in Chickpea (Cicer arietinum L) Role of genotypes, growth regulators and explants Indian Journal of Biotechnology. 4, 251-256.
Boissot, N., aldez, M. V., &
Guiderdoni, E. (1990). Plant regeneration from leaf and seed derived calli and
suspension cultures of the African perennial wild rice, Oryza longistaminata . Plant Cell Report . 9: 447 450.
Borjian, L., & Arak, H. (2013). A
Study on the Effect of Differe nt Concentration of plant hormones (BAP,
NAA, 2, 4 D, and Kinetin) on callus induction in Brassica Napus International Research Journal of Applied and Basic Sciences . 5, 519 521.
Byale, G. K., & Srinath, R.(2013). Effect of Plant Growth Regulators on Orga
nogenesis in Cultivated
Tomato (Mill.) In Vitro Lycopersicon Esculentum . Asian. j. biol. sci . 4, 591 596.
Cearley, T. A.,& Bolyard, M. G. (1997).
Regeneration of Solanum tuberosum cv. Katahdin from leaf
explants in vitro . American Potato Journal . 74, 125 129.
Cheng, Z. J., Wang, L., Sun, W., Zhang, Y., Zhou, C., Su Y. H., Li, W., Sun, T. T., Zhao, X. Y., Li, X. G., Cheng, Y., Zhao, Y., Xie, Q., & Zhang, X. S. (2012). Pattern of Auxin and Cytokinin Responses for Shoot Meristem Induction Results from Regulation of Cytokinin Biosynthesis by Auxin Response Factor 3. Plant Physiology Preview. DOI:10.1104/pp.112.203166. DOI: https://doi.org/10.1104/pp.112.203166
Esna
Ashari, M., & T. A. Villiers. (1998). Plant regeneration from tuber disc of potato ( Solanum tubersom
L .) using 6 benzylaminopurine (6 BAP). Potato Research . 41, 371 382.
Fotso, M., Elian, H. D., Tchouga, A. O., Djeuani, A. C., Mbouobda, H. D., &Omokolo, N. D. (2014).
Effect of exogenous phytohormones and sucrose on micropropagation and microtuberisation of Manihot
esculenta Crantz var. Africain journal of biotechnology .13, 3966 3976.
Gabriele, S., & Hans
George, S. (2003). Effect of genotype on callus induction, shoot regeneration, and
phenotypic stability of regenerated plants in the greenhouse of Primula ssp. Plant Cell, Tissue and Org an
Culture .72, 53 61.
Garcia, E. D., & Martinez ,S. (1995).
Somatic embryogenesis in Solanum tubersom L. cv. Desiree from
stem nodal sections . J. Plant Physiology . 145, 526 530.
Haque, M., I. Mila, N. B.,
Khan ., MS Sarker, R. H. (1996). Shoot regeneration and in vitro microtuberformation in potato (
Solanum tubersom L). Bangladesh journal of botany . 25, 87 93.
Hera, C., Nida, F., & Iffat, Z. A. (2014). Establishment of callus and cell suspension cultures of Nigella Sativa L. for Thymol Production. International Journal of Pharmacy and Pharmaceutical Sciences. 6, 788.
Ighilhariz, Z., Bouabdallah, L., & Belkhodja, M. (2008). Hormonal influence on the induction of callus in
Atriplex Halimus L. L. and Atriplex Canescens (Pursch. European Journal of Scientific Research . 24,
218.
Ishii, Y., Takamura, T
., Goi, M., & M. Tanaka. (2004). Callus induction and somatic embryogenesis of
Phalaenopsis. Plant Cell Report . 17, 446 450.
Jayaree, T. Pavan, U., Ramesh, M., Rao,
A. V., Reddy, J. M. K., & Sadanandam, A. (2001). Somatic
embryogenesis from leaf culture of potato. Plant Cell Tissue Organ Culture . 64, 13 17.
Juan C, Ritieni, A., &
Mañes, J. (2012) Determination of trichothecenes and zearalenones in grain cereal,
flour and bread by liquid chromatography tandem mass spectrometry. Food Chemistry , 134(4): 2389 97 DOI: https://doi.org/10.1016/j.foodchem.2012.04.051
Kaur,
M., Kaur, R., Sharma, C., Kaur, N., & A. Kaur (2014). Effect of gr owth regulators on micro
propagation of potato cultivars. Journal of Cell and Tissue Research . 14, 4363 4366.
Kha
diga, G. A., Rasheid, S. M., & Mutasim, K. (2009). Effect of plant growth regulators on callus
induction and plant regeneration in tuber segme nt culture of potato ( Solanum tuberosum L.) cultivar
Diamant . African Journal of Biotechnology . 8, 2529 2534.
Koutoua, A., Houda, EY., & R. Atmane. (2007). 2,4
D influence on embryonic callus explants Wheat. Bull.
Soc. Pharm. Bordeaux .146, 97 112.
Leyser,
O. (2001). Auxin signalling: The beginning, the middle and the end. Current Opinion in Plant
Biology . 4, 382 386.
Majid, I., Muhammad, J., Rizwan, R., Syed, Z U., Muhammad, S I., Misbah, R., & Salman, M. (2014). Effect of Plant Growth Regulators on Callus Formation. Potato Journal of Agri-Food and Applied Sciences. 2, 77-81.
Manel, A.S., Malabika, R.
P., Ahmed, A., Mohamed, A., & Assma, A. (2014). Effect of plant growth
regulators on the regeneration of endangered plant Calligonum colosum L Henry in the kingdom of
Bahrain . African journal of biotechnology . 13, 2513 2523.
Maryam, A
., Mohammad, G., Reza, M., & Fath Ali, N. (2013). Effect of Different Concentrations of 2,4 D
on Callus Induction and Callus root Indication in 2 Explants from True Potato ( Sola num tuberosum L.)
Seeds. Scientific Journal of Agronomy and Plant Breeding .1, 56 63.
Mohammad, B.
Y., Mansour, G., Mahmoud, E. A., & Hussein, k. (2014). Effect of season and explants type
on in vitro propagation of Juglansregia L.genotypes Z60 and Z63. Int ernational Journal of Agriculture and
Crop Sciences . 7 9, 624 629.
Mozafri, J., Wolyn, D. J., & Ali Khan, S. T. (1997). Chromosome doubling via tuber disc culture in
dihaploid potato as determined by confocal microscopy. Plant Cell. Rep . 16, 329 333. DOI: https://doi.org/10.1007/BF01088291
Mutasim, M. K., Khadiga, G. A., & Rasheid, S. M. (2010). Callus formation and organogenesis of potato
Solanum tuberosum L) cultivar Almeriz. Journal of Phytology. 2, 40 4.
Nistor, A., Câmpeanu, Gh., Nicoleta, Ch., & Diana, K. (2009). Effect of auxine an d cytokinine on callus
Induction in potato ( Solanum tuberosum L.) Explants. Agricultura Stiintasi Practica . 1 2, 69 70.Pretova, A., & Dedicova, B. (1992). Somatic embryogenesis in
Solanum tubersom L. cv. Desiree from
unripe zygotic embryos. Journal of Plant Physiol . 139, 539 542.
Qamar, L. A., Bouchra, C., & C. Hassan.
. Callogenèse et régénération in vitro du kiwi ( Actinidia
chinensis ) à partir de divers explant. Actes Inst. Agron. Vet . 21, 75 81.
Renu., S & Nidhi, B. (2011). Impact of growth reg
ulators on Callus production of two medicinal plants viz.
Adhatoda vasica and Ageratum conyzoides. International Journal of Research in Plant Science . 1, 1 8.
Ruck, A., Palme, K., Venis, M. A., Napier, RM., & Felleh, H. (1993).
Patch clamp analysis establi shes a
role for an auxin binding protein in the auxin stimulation of plasma membrane current in Zea mays
protoplasts. Plant Journal . 4, 41 46.
Shahab
ud din., Sultan, I. N., kakar, M.A., Yousafzai, A., Sattar, F. A., Ahmmad , F., Ibrahim, S. M.,
Hassanulla h, M., & Arif, B. (2011). The Effects of Different Concentrations and Combinations of Growth
Regulators on the Callus Formation of Potato ( Solanum tubrosum ) Explants. Current Research Journal of
Biological Sciences .3, 499 503.
Shirin, F., Hossain, M., Kab
ir, M. F., Roy, M. & Sarker S. R. (2007). Callus Induction and Plant
Regeneration from Internodal and Leaf Explants of Four Potato ( Solanum tuberosum L.) cultivars. World
Journal. Agricultural. Science. 3, 01 06.
Solmon
Blackburn, R. M., & Baker, H. (2001). Breeding resistance virus potatoes Solanum tuberosum L)
a review of traditional and molecular approaches. Heridity . 6, 17 35.
Teresa, E.V., Eva, D. G., & O. Maira. (2005). Somatic embryogenesis in Solanum tuberosum from cell
suspension cultures: Histological analysis and extracellular protein patterns. Journal of Plant Physiology.
, 449 456.
Udha ya, A., Peter, G. A., and Arun, N. (2012). Effect of growth hormones on callus induction of Sauropus
androgynous (Sweet Annals of Biological R esearch . 3, 4668 4674.
Vaishali, B., Maninder, S., Deepti, S., Jitendra,M., & Sudershan, R. (2013).
Induction of different types of
callus and somatic embryogenesis in various explants of Sainfoin ( Onobrychis sativa. International journal
of recent biote chnology. 1, 1 8.
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