Safety profile and prevention of cognitive deficit in alzheimer’s disease model of graphene family nanomaterials, Tucuma oil (Astrocaryum Vulgare) and its synergisms
DOI:
https://doi.org/10.31686/ijier.vol10.iss3.3694Keywords:
cell viability, computational simulations, graphene oxide, nanotoxicology, reduced graphene oxide, memoryAbstract
Alzheimer's disease is a worldwide health issue, and there are currently no treatments that can stop this disease. Oxidized graphene derivatives have gained prominence in use in biological systems due to their excellent physical-chemical characteristics, biocompatibility and ability to overcome the blood-brain barrier. Other substances highlighted are those of natural origin from the Amazon biome, such as tucuma, a fruit whose oil has been widely studied in therapeutic applications. Thus, the aim of this study was to investigate the action of graphene oxide, reduced graphene oxide and tucuma oil, isolated and combined, as an alternative for treatment of Alzheimer's disease through studies in silico, in vitro, in vivo and ex vivo. Computational simulation via docking was used to verify the affinity of the substances with the proteins β-amyloid and acetylcholinesterase, in which the reduced graphene oxide was the one that showed the most favorable interaction. The results of the ab initio simulation showed that the synergism between the nanostructures and the oil occurs through physical adsorption. The experimental results revealed that the substances and their combinations were nontoxic, both at the cellular and systemic level. In general, all treatments had positive results against induced memory deficit, but reduced graphene oxide was the most prominent, as it was able to protect against memory damage in all behavioral tests performed, with anticholinesterase activity and antioxidant effect. In conclusion, the reduced graphene oxide is, among the treatments studied, the one with great therapeutic potential to be investigated in the treatment of this disease.
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Copyright (c) 2022 Patricia Ferreira Schopf, Mikaela Peglow Pinz, Ketlyn Pereira da Motta, Vitor Pereira Klein, Alencar Kolinski Machado, Cristiano Rodrigo Bohn Rhoden, Ethel Antunes Wilhelm, Cristiane Luchese, Ivana Zanella, Michele Sagrillo
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Accepted 2022-03-06
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