In Vitro Antioxidant Potential of Baccharis trimera and Baccharis articulata Infusions in Postmenopausal Women
DOI:
https://doi.org/10.31686/ijier.vol6.iss7.1060Keywords:
Postmenopausal, Oxidative Stress, Carqueja, AntioxidantAbstract
Considering that postmenopausal women generally have high levels of oxidative markers and low levels of antioxidant defense markers due to the reduction of estrogen levels, and because of the vast popular use of several species of carja in the form of infusion, this study aimed to evaluate in vitro if infusions of B. trimera and B. articulata have antioxidant potential in erythrocytes of postmenopausal women and which are the most effective. The erythrocytes from 40 postmenopausal women were treated in vitro for 1 hour with infusions of B. trimera and B. articulata at the following concentrations: 4, 8, 16, 33, 66 g/L. The negative control consisted of erythrocytes from postmenopausal women without treatment with the plants. After treatment, the levels of thiobarbituric acid reactive substances (TBARS), carbonylated proteins (CP), and reduced glutathione (GSH) were measured. The infusions of B. trimera and B. articulata at concentrations of 33 (p<0.001) and 66 g/L (p<0.001) reduced the level of TBARS in comparison to the negative control, and the effect size (ES) for this reduction was small. The levels of GSH increased after treating with B. trimera infusion at a concentration of 66 (p<0.001) and with B. articulata at concentrations of 33 (p<0.001) and 66 g/L (p<0.001), when compared with the negative control, and the ES for this increase was average. The infusions of B. trimera and B. articulata show antioxidant potential in vitro, thus showing a similar effect with regards to the reduction of oxidative damage to lipids and increased endogenous antioxidant protection.
References
Alonso, J., and Desmarchelier, C. 2006. Plantas medicinales autóctonas de la Argentina: bases científicas para su aplicación en atención primaria de la salud. Buenos Aires: LOLA, 663p.
Borgo, J., Xavier, C.A.G., Moura, D.J., Richter, M.F., Suyenaga, E.S. 2010. The Influence of drying processes on flavonoid level and the antioxidant activity of Baccharis articulata (Lam.) Pers extracts. Rev Bras Farmacogn, 20(1):12-17. DOI: 10.1590/S0102-695X2010000100004
Borrelli, F., and Edzard, E. 2010. Alternative and complementary therapies for the menopause. Maturitas, 66(4):333-343. DOI: 10.1016/j.maturitas.2010.05.010
Brasil. Agência Nacional de Vigilância Sanitária. 2011. Formulário de Fitoterápicos da Farmacopeia Brasileira. Brasília, 126p.
Butterfield, D.A., and Dalle-Donne, I. 2012. Redox proteomics. Antioxid Redox Signal, 17(11):1487-1489. DOI: 10.1089/ars.2012.4742
Campos, F.R., Bressan, J., Jasinski, V.C.G., Zuccolotto, T., Da Silva, L.E., Cerqueira, L.B. 2016. Baccharis (Asteraceae): chemical constituents and biological activities. Chem Biodivers, 13(1):1-17. DOI: 10.1002/cbdv.201400363
Catalgol, B.K., Ozden, S., Alpertunga, B. 2007. Effect of trichlorfon on molondialdehyde and antixidant system in human erythrocytes. Toxicol In Vitro, 21(8):1538-1544. DOI: 10.1016/j.tiv.2007.06.002
Chandra, S., and Mejia, E.G. 2004. Polyphenolic compounds, antioxidant capacity and quinone reductase activity of na aqueous extract of Ardisia compressa in comparison to mate (Ilex paraguariensis) and green teas (Camellia sinensis). J Agric Food Chem, 52(11):3583-3589. DOI: 10.1021/jf0352632
Circu, M.L., and Aw, T.Y. 2010. Reactive oxygen species, cellular redox systems, and apoptosis. Free Radic Biol Med, 48(6):749-762. DOI: 10.1016/j.freeradbiomed.2009.12.022
Cohen, J. 1988. Statistical power analysis for the behavioral sciences. 2nded. Hillsdale, NJ: Erlbaum.
Davies , K.J. 2001. Degradation of oxidized proteins by the 20S proteasome. Biochimie,83(3-4):301-310. DOI: 10.1016/S0300-9084(01)01250-0
De Oliveira, C.B., Comunello, L.N., Lunardelli, A., Amaral, R.H., Pires, M.G., Da Silva, G.L., Manfredini, V., Vargas, C.R, Gnoatto, S.C., De Oliveira, J.R., Gosmann, G. 2012. Phenolic enriched extract of Baccharis trimera presents antiinflammatory and antioxidant activities. Molecules, 17(1):1113-1123. DOI: 10.3390/molecules17011113
De Oliveira, S.Q., Dal-Pizzol, F., Gosmann, G., Guillaume, D., Moreira, J.C., Schenkel, E.P. 2003. Antioxidant activity of Baccharis articulata extracts: isolation of a new compound with antioxidant activity. Free Radic Res, 37(5):555-559. DOI: 10.1080/1071576031000076259
Dias, L.F.T., De Melo, E.S., Hernandes, L.S., Bacchi, E.M. 2009. Antiulcerogenic and antioxidant activities of Baccharis trimera (Less) DC (Asteraceae). Rev Bras Farmacogn, 19(1B):309-314. DOI: 10.1590/S0102-695X2009000200022
Doshi, S.B., and Agarwal, A. 2013. The role of oxidative stress in menopause. J Midlife Health, 4(3):140-146. DOI: 10.4103/0976-7800.118990
Ellman, G.L. 1959. Tissue sulfhydryl groups. Arch Biochem Biophys, 82(1):70-77. DOI: 10.1016/0003-9861(59)90090-6
Gelatti, G.T., De Oliveira, K.R., Colet, C.F. 2016. Potential drug interactions in relation with the use, medicine plants and herbal in premenopausal women period. J Res: Fundam Care, 8(2):4328-4346. DOI: 10.9789/2175-5361.2016.v8i2.4328-4346
Höhn, A., König, J., Grune, T. 2013. Protein oxidation in aging and the removal of oxidized proteins. J Proteomics, 92:132-159. DOI: 10.1016/j.jprot.2013.01.004
Koury, J.C., and Donangelo, C.M. 2003. Zinc, oxidative stress and physical activity. Rev Nutr, 16(4):433-441. DOI: 10.1590/S1415-52732003000400007
Kumar, P., and Maurya, P.K. 2013. L-cysteine efflux in erythrocytes as a function of human age: correlation with reduced glutathione and total anti-oxidant potential. Rejuvenation Res, 16(3):179-184. DOI: 10.1089/rej.2012.1394
Lasserre, A., and Fournier, A. 2016. Menopausal hormonal therapy and cancer risks. Gynecol Obstet Fertil, 44(7-8):424-427. DOI: 10.1016/j.gyobfe.2016.05.012
Lee, C.H., Cheng, C.L., Kao Yang, Y.H., Lin, J. 2015. Hormone therapy and risk of venous thromboembolism among postmenopausal women in Taiwan - a 10 year nationwide population-based study. Circ J, 79(5):1107-1114. DOI: 10.1253/circj.CJ-14-1227
Levine, R.L., Garland, D., Oliver, C.N., Amici, A., Climent, I., Lenz, A.G., Ahn, B.W., Shaltiel, S., Stadtman, E.R. 1990. Determination of carbonyl content in oxidatively modified proteins. Methods Enzymol, 186:464-478. DOI: 10.1016/0076-6879(90)86141-H.
Maurya, P.K., Kumar, P., Chandra, P. 2015. Biomarkers of oxidative stress in erythrocytes as a function of human age. World J Methodol, 5(4):216-222. DOI: 10.5662/wjm.v5.i4.216
Mendes, F.R., Tabach, R., Carlini, E.A. 2007. Evaluation of Baccharis trimera and Davilla rugosa in tests for adaptogen activit. Phytother Res, 21(6):517-522. DOI: 10.1002/ptr.2080
Morrison, I.M., Asiedu, E.A., Stuchbury, T., Powell, A.A. 1995. Determination of lignin and tannin contents of Cowpea seed coats. Ann Bot, 76(3):287-290. DOI: 10.1006/anbo.1995.1097
NAMS. Sociedade Norte-Americana de Menopausa. Guia da Menopausa. 7.ed. 2013. 87p.
Ogunro, P.S., Bolarinde, A.A., Owa, O.O., Salawu, A.A., Oshodi, A.A. 2014. Antioxidant status and reproductive hormones in women during reproductive, perimenopausal and postmenopausal phase of life. Afr J Med Med Sci, 43(1):49-57.
Oliveira, S.Q., Dal-Pizzol, F., Moreira, J.C.F., Schenkel, E.P., Gosmann, G. 2004. Antioxidant activity of Baccharis spicata, Baccharis trimera and Baccharis usterii. Acta Farm Bonaer, 23(3):365-368.
Pádua, B.C., Rossoni Júnior, J.V., Magalhães, C.L.B., Chaves, M.M., Silva, M.E., Pedrosa, M.L., De Souza, G.H.B., Brandão, G.C., Ivanildes Vasconcelos Rodrigues, I.V., Lima, W.G., Costa, D.C. 2014. Protective effect of Baccharis trimera extract on acute hepatic injury in a model of inflammation induced by acetaminophen. Mediators Inflamm, 2014:1-14. DOI: 10.1155/2014/196598
Pompella, A., Visvikis, A., Paolicchi, A., De Tata, V., Casini, A.F. 2003. The changing faces of glutathione, a cellular protagonist. Biochem Pharmacol, 66(8):1499-1503. DOI: 10.1016/S0006-2952(03)00504-5 63
Rates, S.M.K. 2001. Promoção do uso racional de fitoterápicos: uma abordagem no ensino de farmacognosia. Rev Bras Farmacogn, 11(2):57-69. DOI: 10.1590/S0102-695X2001000200001
Sies, H. 2015. Oxidative stress: a concept in redox biology and medicine. Redox Biol, 4:180-183. DOI: 10.1016/j.redox.2015.01.002
Signorelli, S.S., Neri, S., Sciacchitano, S., Pino, L.D., Costa, M.P., Marchese, G., Celotta, G., Cassibba, N., Pennisi, G., Cashetto, S. 2006. Behaviour of some indicators of oxidative stress in postmenopausal and fertile women. Maturitas, 53(1):77-82. DOI: 10.1016/j.maturitas.2005.03.001
Simões, C.M.O, Schenkel, E.P., De Mello, J.C.P., Mentz, L.A., Petrovick, P.R. 2017. Farmacognosia: do produto natural ao medicamento. 1.ed. Artmed, 502p.
Simões-Pires, C.A., Queiroz, E.F., Henriques, A.T., Hostettmann, K. 2005. Isolation and on-line identification of antioxidant compounds from three Baccharis species by HPLC-UVMS/MS with post-column derivatisation. Phytochem Anal, 16(5):307-314. DOI: 10.1002.pca.826
Stocks, J., and Dormandy, T.L. 1971. The autooxidation of human red cell lipids induced by hydrogen peroxide. Br J Haematol, 20(1):95-111. DOI: 10.1111/j.1365-2141.1971.tb00790.x
Umesh, C.S.Y., and Ramana, K.V. 2013. Regulation of NF-
Borgo, J., Xavier, C.A.G., Moura, D.J., Richter, M.F., Suyenaga, E.S. 2010. The Influence of drying processes on flavonoid level and the antioxidant activity of Baccharis articulata (Lam.) Pers extracts. Rev Bras Farmacogn, 20(1):12-17. DOI: 10.1590/S0102-695X2010000100004
Borrelli, F., and Edzard, E. 2010. Alternative and complementary therapies for the menopause. Maturitas, 66(4):333-343. DOI: 10.1016/j.maturitas.2010.05.010
Brasil. Agência Nacional de Vigilância Sanitária. 2011. Formulário de Fitoterápicos da Farmacopeia Brasileira. Brasília, 126p.
Butterfield, D.A., and Dalle-Donne, I. 2012. Redox proteomics. Antioxid Redox Signal, 17(11):1487-1489. DOI: 10.1089/ars.2012.4742
Campos, F.R., Bressan, J., Jasinski, V.C.G., Zuccolotto, T., Da Silva, L.E., Cerqueira, L.B. 2016. Baccharis (Asteraceae): chemical constituents and biological activities. Chem Biodivers, 13(1):1-17. DOI: 10.1002/cbdv.201400363
Catalgol, B.K., Ozden, S., Alpertunga, B. 2007. Effect of trichlorfon on molondialdehyde and antixidant system in human erythrocytes. Toxicol In Vitro, 21(8):1538-1544. DOI: 10.1016/j.tiv.2007.06.002
Chandra, S., and Mejia, E.G. 2004. Polyphenolic compounds, antioxidant capacity and quinone reductase activity of na aqueous extract of Ardisia compressa in comparison to mate (Ilex paraguariensis) and green teas (Camellia sinensis). J Agric Food Chem, 52(11):3583-3589. DOI: 10.1021/jf0352632
Circu, M.L., and Aw, T.Y. 2010. Reactive oxygen species, cellular redox systems, and apoptosis. Free Radic Biol Med, 48(6):749-762. DOI: 10.1016/j.freeradbiomed.2009.12.022
Cohen, J. 1988. Statistical power analysis for the behavioral sciences. 2nded. Hillsdale, NJ: Erlbaum.
Davies , K.J. 2001. Degradation of oxidized proteins by the 20S proteasome. Biochimie,83(3-4):301-310. DOI: 10.1016/S0300-9084(01)01250-0
De Oliveira, C.B., Comunello, L.N., Lunardelli, A., Amaral, R.H., Pires, M.G., Da Silva, G.L., Manfredini, V., Vargas, C.R, Gnoatto, S.C., De Oliveira, J.R., Gosmann, G. 2012. Phenolic enriched extract of Baccharis trimera presents antiinflammatory and antioxidant activities. Molecules, 17(1):1113-1123. DOI: 10.3390/molecules17011113
De Oliveira, S.Q., Dal-Pizzol, F., Gosmann, G., Guillaume, D., Moreira, J.C., Schenkel, E.P. 2003. Antioxidant activity of Baccharis articulata extracts: isolation of a new compound with antioxidant activity. Free Radic Res, 37(5):555-559. DOI: 10.1080/1071576031000076259
Dias, L.F.T., De Melo, E.S., Hernandes, L.S., Bacchi, E.M. 2009. Antiulcerogenic and antioxidant activities of Baccharis trimera (Less) DC (Asteraceae). Rev Bras Farmacogn, 19(1B):309-314. DOI: 10.1590/S0102-695X2009000200022
Doshi, S.B., and Agarwal, A. 2013. The role of oxidative stress in menopause. J Midlife Health, 4(3):140-146. DOI: 10.4103/0976-7800.118990
Ellman, G.L. 1959. Tissue sulfhydryl groups. Arch Biochem Biophys, 82(1):70-77. DOI: 10.1016/0003-9861(59)90090-6
Gelatti, G.T., De Oliveira, K.R., Colet, C.F. 2016. Potential drug interactions in relation with the use, medicine plants and herbal in premenopausal women period. J Res: Fundam Care, 8(2):4328-4346. DOI: 10.9789/2175-5361.2016.v8i2.4328-4346
Höhn, A., König, J., Grune, T. 2013. Protein oxidation in aging and the removal of oxidized proteins. J Proteomics, 92:132-159. DOI: 10.1016/j.jprot.2013.01.004
Koury, J.C., and Donangelo, C.M. 2003. Zinc, oxidative stress and physical activity. Rev Nutr, 16(4):433-441. DOI: 10.1590/S1415-52732003000400007
Kumar, P., and Maurya, P.K. 2013. L-cysteine efflux in erythrocytes as a function of human age: correlation with reduced glutathione and total anti-oxidant potential. Rejuvenation Res, 16(3):179-184. DOI: 10.1089/rej.2012.1394
Lasserre, A., and Fournier, A. 2016. Menopausal hormonal therapy and cancer risks. Gynecol Obstet Fertil, 44(7-8):424-427. DOI: 10.1016/j.gyobfe.2016.05.012
Lee, C.H., Cheng, C.L., Kao Yang, Y.H., Lin, J. 2015. Hormone therapy and risk of venous thromboembolism among postmenopausal women in Taiwan - a 10 year nationwide population-based study. Circ J, 79(5):1107-1114. DOI: 10.1253/circj.CJ-14-1227
Levine, R.L., Garland, D., Oliver, C.N., Amici, A., Climent, I., Lenz, A.G., Ahn, B.W., Shaltiel, S., Stadtman, E.R. 1990. Determination of carbonyl content in oxidatively modified proteins. Methods Enzymol, 186:464-478. DOI: 10.1016/0076-6879(90)86141-H.
Maurya, P.K., Kumar, P., Chandra, P. 2015. Biomarkers of oxidative stress in erythrocytes as a function of human age. World J Methodol, 5(4):216-222. DOI: 10.5662/wjm.v5.i4.216
Mendes, F.R., Tabach, R., Carlini, E.A. 2007. Evaluation of Baccharis trimera and Davilla rugosa in tests for adaptogen activit. Phytother Res, 21(6):517-522. DOI: 10.1002/ptr.2080
Morrison, I.M., Asiedu, E.A., Stuchbury, T., Powell, A.A. 1995. Determination of lignin and tannin contents of Cowpea seed coats. Ann Bot, 76(3):287-290. DOI: 10.1006/anbo.1995.1097
NAMS. Sociedade Norte-Americana de Menopausa. Guia da Menopausa. 7.ed. 2013. 87p.
Ogunro, P.S., Bolarinde, A.A., Owa, O.O., Salawu, A.A., Oshodi, A.A. 2014. Antioxidant status and reproductive hormones in women during reproductive, perimenopausal and postmenopausal phase of life. Afr J Med Med Sci, 43(1):49-57.
Oliveira, S.Q., Dal-Pizzol, F., Moreira, J.C.F., Schenkel, E.P., Gosmann, G. 2004. Antioxidant activity of Baccharis spicata, Baccharis trimera and Baccharis usterii. Acta Farm Bonaer, 23(3):365-368.
Pádua, B.C., Rossoni Júnior, J.V., Magalhães, C.L.B., Chaves, M.M., Silva, M.E., Pedrosa, M.L., De Souza, G.H.B., Brandão, G.C., Ivanildes Vasconcelos Rodrigues, I.V., Lima, W.G., Costa, D.C. 2014. Protective effect of Baccharis trimera extract on acute hepatic injury in a model of inflammation induced by acetaminophen. Mediators Inflamm, 2014:1-14. DOI: 10.1155/2014/196598
Pompella, A., Visvikis, A., Paolicchi, A., De Tata, V., Casini, A.F. 2003. The changing faces of glutathione, a cellular protagonist. Biochem Pharmacol, 66(8):1499-1503. DOI: 10.1016/S0006-2952(03)00504-5 63
Rates, S.M.K. 2001. Promoção do uso racional de fitoterápicos: uma abordagem no ensino de farmacognosia. Rev Bras Farmacogn, 11(2):57-69. DOI: 10.1590/S0102-695X2001000200001
Sies, H. 2015. Oxidative stress: a concept in redox biology and medicine. Redox Biol, 4:180-183. DOI: 10.1016/j.redox.2015.01.002
Signorelli, S.S., Neri, S., Sciacchitano, S., Pino, L.D., Costa, M.P., Marchese, G., Celotta, G., Cassibba, N., Pennisi, G., Cashetto, S. 2006. Behaviour of some indicators of oxidative stress in postmenopausal and fertile women. Maturitas, 53(1):77-82. DOI: 10.1016/j.maturitas.2005.03.001
Simões, C.M.O, Schenkel, E.P., De Mello, J.C.P., Mentz, L.A., Petrovick, P.R. 2017. Farmacognosia: do produto natural ao medicamento. 1.ed. Artmed, 502p.
Simões-Pires, C.A., Queiroz, E.F., Henriques, A.T., Hostettmann, K. 2005. Isolation and on-line identification of antioxidant compounds from three Baccharis species by HPLC-UVMS/MS with post-column derivatisation. Phytochem Anal, 16(5):307-314. DOI: 10.1002.pca.826
Stocks, J., and Dormandy, T.L. 1971. The autooxidation of human red cell lipids induced by hydrogen peroxide. Br J Haematol, 20(1):95-111. DOI: 10.1111/j.1365-2141.1971.tb00790.x
Umesh, C.S.Y., and Ramana, K.V. 2013. Regulation of NF-
Downloads
- PDF 147 200
Published
2018-07-01
Issue
Section
Journal Articles
License
Copyright (c) 2018 Gabriela Tassotti Gelatti, Ana Caroline Tissiani, Mariana Spanamberg Mayer, Tamiris Felippin, Daiana Meggiolaro Gewehr, Jana Koefender, Evelise Moraes Berlezi, Diego Pascoal Golle, Roberta Cattaneo Horn
This work is licensed under a Creative Commons Attribution-NoDerivatives 4.0 International License.
Copyrights for articles published in IJIER journals are retained by the authors, with first publication rights granted to the journal. The journal/publisher is not responsible for subsequent uses of the work. It is the author's responsibility to bring an infringement action if so desired by the author for more visit Copyright & License.
How to Cite
Gelatti, G. T., Tissiani, A. C., Mayer, M. S., Felippin, T., Gewehr, D. M., Koefender, J., Berlezi, E. M., Golle, D. P., & Horn, R. C. (2018). In Vitro Antioxidant Potential of Baccharis trimera and Baccharis articulata Infusions in Postmenopausal Women. International Journal for Innovation Education and Research, 6(7), 99-112. https://doi.org/10.31686/ijier.vol6.iss7.1060
Share
Most read articles by the same author(s)
- André Schoffel, Sidinei José Lopes, Jana Koefender, Alessandro Dal'Col Lúcio, Juliane Nicolodi Camera, Diego Pascoal Golle, Sample size for estimation of averages of agronomic traits in cassava seedlings , International Journal for Innovation Education and Research: Vol. 8 No. 5 (2020): International Journal for Innovation Education and Research
- Gilmar Seidel, Caroline Wesp, Jana Koefender, Diego Pascoal Golle, André Schoffel, Elaine Deuner, Juliane Nicolodi Camera, Fungicides application in corn disease control and mycotoxin accumulation in grain , International Journal for Innovation Education and Research: Vol. 8 No. 8 (2020): International Journal for Innovation Education and Research
- Roberta Cattaneo Horn, GABRIELA TASSOTTI GELATTI, JANA KOEFENDER, DIEGO PASCOAL GOLLE, MARIANA SPANEMBERG MAYER, TIAGO ANTONIO HERINGER, MAURICIO BATISTELLA PASINI, ANA CAROLINE TISSIANI, AIME CUNHA ARRUDA, LAURA RUBIN, RAFAELA DA ROSA RECKTENWALD, PAULO RICARDO MOREIRA, CAROLINE ALEGRANSI, Antioxidant effect of Physalis angulata fruit on cells exposed to 2,4- dichlorophenoxyacetic acid , International Journal for Innovation Education and Research: Vol. 7 No. 8 (2019): International Journal for Innovation Education and Research
- Rafaela Muraro, Nadiel Kist, Mauricio Paulo Batistella Pasini, Jana Koefender, Rafael Pivotto Bortolotto, André Schoffel, Luana Carvalhaes Coutinho , Juliane Camera, Use of isolated fungicides for the control of Phakopsora pachyrhizi in soybean , International Journal for Innovation Education and Research: Vol. 8 No. 3 (2020): International Journal for Innovation Education and Research
- Luana Carvalhaes Coutinho, Juliane Nicolodi Camera, André Schoffel, Diego Pascoal Golle, João Fernando Zamberlan, Theodoro Schneider, Carolina Cardoso Deuner, Jana Koefender, Péricles Delazeri, Seed cassava cuttings production , International Journal for Innovation Education and Research: Vol. 8 No. 3 (2020): International Journal for Innovation Education and Research
- Juliane Nicolodi Camera, Jana Koefender, Diego Pascoal Golle, Roberta Cattaneo Horn, Igor Pirez Valério, Eduardo Fiorin Flores, Carolina Cardoso Deuner, Efficiency of fungicide chemical group in the preventive and curative control of Puccinia sorghi in corn and Cercospora zeae-maydis sporulation in different culture media , International Journal for Innovation Education and Research: Vol. 6 No. 9 (2018): International Journal for Innovation Education and Research
- Rodrigo Fernando dos Santos Salazar, Thaís Cristina Coelho de Ornelas Salasar, Roberta Cattaneo Horn, João Fernando Zamberlan, Rafael Pivotto Bortolotto, Kelly de Moura Oliveira Krause, Analysis of Teaching Strategies and Perception of Nursing Scholars at Risk Communication to Workers in Situations of Social and Economic Risk , International Journal for Innovation Education and Research: Vol. 7 No. 9 (2019): International Journal for Innovation Education and Research
- Juliane Nicolodi Camera, Valéria Cecília Ghissi , Jana Koefender, André Schoffel, Pedro Luiz Martins Soares, Carolina Cardoso Deuner, Population survey of phytonematodes in soybean crop in the Middle Plateau region in Rio Grande do Sul , International Journal for Innovation Education and Research: Vol. 7 No. 9 (2019): International Journal for Innovation Education and Research
- José Luis Perlin, Dieison Prestes da Silveira, Jana Koefender, Roberta Cattaneo Horn, Juliane Nicolodi Camera, Vania Abreu de Oliveira, Patrícia Dall’Agnol Bianchi, Diego Pascoal Golle, The Analysis of Water Availability as an Important Factor for Farmers' Permanence in the Field: A Study in Southern Brazilian Countryside , International Journal for Innovation Education and Research: Vol. 7 No. 10 (2019): International Journal for Innovation Education and Research
- Matheus Alegretti de Oliveira , Mario Antônio Bianchi, Jana Koefender, Theodoro Schneider, André Schoffel, Juliane Nicolodi Camera, Diego Pascoal Golle, Herbicide resistance technologies in soybean cultivars , International Journal for Innovation Education and Research: Vol. 10 No. 3 (2022): International Journal for Innovation Education and Research