Inoculation with plant growth-promoting bacteria and reduction of nitrogen fertilizer in herbage accumulation and nutritional value of Mavuno grass

Amário Nuno Meireles Duarte; Cecílio Viega Soares Filho; Marcelo Carvalho Minhoto Teixeira Filho; Caroline Lopes Monteiro de Carvalho; Mariangela Hungria; MARCO ANTONIO NOGUEIRA; Isadora Mandolesi Valvano; Amanda Gonçalves Ishiy

doi:10.31686/ijier.vol9.iss3.2962

Authors

DUARTE, A. N. M. Sao Paulo State University http://orcid.org/0000-0002-9121-6848
SOARES FILHO, C. V. 1São Paulo State University (UNESP), Araçatuba, SP. https://orcid.org/0000-0003-1585-5450
TEIXEIRA FILHO, M. C. M. 1São Paulo State University (UNESP), Ilha Solteira, SP. https://orcid.org/0000-0003-2303-3465
CARVALHO, C. L. M. de 1São Paulo State University (UNESP), Araçatuba, SP. http://orcid.org/0000-0002-7723-4900
HUNGRIA, M. Embrapa-Soja, CNPMSo, Londrina, PR, Brazil https://orcid.org/0000-0002-5132-8685
NOGUEIRA, M. A. Embrapa-Soja, CNPMSo, Londrina, PR, Brazil https://orcid.org/0000-0001-7747-9839
VALVANO, I. M. 1São Paulo State University (UNESP), Araçatuba, SP. http://orcid.org/0000-0003-1778-0472
ISHIY, A. G. 1São Paulo State University (UNESP), Araçatuba, SP. https://orcid.org/0000-0002-7021-2856

DOI:

https://doi.org/10.31686/ijier.vol9.iss3.2962

Keywords:

crude protein, tropical forage grass, diazotrophic bacteria, Urochloa spp

Abstract

Strategies that improve the use of nitrogen (N), by tropical forage grasses, can bring environmental and social gains. Thus, this study aimed to evaluate the forage productivity and the nutritional value (NV) of the hybrid Urochloa spp. cv. ´Mavuno`, under inoculation with plant growth-promoting bacteria (PGPB) and doses of N. The experiment was carried out in the field, for a period of 14 months, in a randomized block design, with nine treatments and four replications in plots of 9 m². Seed inoculations were tested with: Azospirillum brasilense Ab-V5 and Ab-V6 strains; Pseudomonas fluorescens CCTB 03 and co-inoculation with Rhizobium tropici CIAT 899 and A. brasilense Ab-V6, combined with 50 and 100 kg ha^-1 of added mineral N; fertilization with N-mineral with these doses, without inoculation and the control treatment, without N and without inoculation. The bacteria were inoculated to the grass seeds. The forage was evaluated by determining the shoot dry weight yield (SDWY) and the levels of crude protein (CP), neutral detergent insoluble fiber (NDF), acid detergent insoluble fiber (ADF) and in vitro dry matter digestibility (IVDMD). The results were subjected to analysis of variance by the F test (p ≤ 0.05) and compared to each other by the Scott-Knott test at 5% probability. Inoculation with PGPB resulted in greater SDWY when associated with a dose of 50 kg ha^-1 of N. In plants inoculated with A. brasilense Ab-V5 + Ab-V6 and P. fluorescens CCTB 03, associated with a dose of 50 kg ha^-1 of N-mineral, SDWY was similar to that obtained with double the dose of N in the absence of inoculation. The inoculation with PGPB resulted in an increase in the NV of Mavuno grass, with emphasis on A. brasilense Ab-V5 + Ab-V6 and P. fluorescens CCTB 03, associated with the dose of 100 kg ha^-1 of N-mineral that improved the nutritional value in all evaluated items. The inoculation with A. brasilense Ab-V5 + Ab-V6 was the most promising, followed by P. fluorescens CCTB 03, mainly in association with 50 kg ha^-1 of N.

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References

ABIEC. 2020. Associação Brasileira das Indústrias Exportadoras de Carne. Beef report 2020 – Perfil da Pecuária do Brasil. Available in http://abiec.com.br/publicacoes/beef-report-2020/. Acessed on august 16th. 2020.

EMBRAPA. 2018. EMPRESA BRASILEIRA DE PESQUISA AGROPECUÁRIA. Quantificação territorial da ocupação e uso de terras no Brasil. Available in https://www.embrapa.br/car/sintese. Acessed on august 15th. 2020.

Costa, K. A. de P., Oliveira, I. P. de, Faquin, V. Adubação nitrogenada para pastagens do gênero Brachiaria em solos do Cerrado. Embrapa Arroz e Feijão-Documentos (INFOTECA-E), 2006. 60p. (Documentos, 192).

Heinrichs, R., Soares Filho, C. V., Crociolli, C. A., Rodrigues, T. A. Doses e fontes nitrogenadas e seus efeitos nos atributos químicos do solo e na produção de forragem da Brachiaria brizantha cv. Xaraés. Ciências Agrárias, Londrina. v. 33, n. 5, p. 1745-1754, 2012. DOI: https://doi.org/10.5433/1679-0359.2012v33n5p1745

Soares Filho, C. V., Cecato, U., Ribeiro, O. L., Roma, C. F. D. C., Beloni, T. Morphogenesis in pastures with Tanzania grass fertilized with nitrogen doses under a grazing system. Acta Scientiarum. Animal Sciences, v. 37, n. 3, p. 235-241, 2015. DOI: https://doi.org/10.4025/actascianimsci.v37i3.27101

Lima, G. C. De, Hungria, M., Nogueira, M. A., Teixeira Filho, M. C. M., Moreira, A., Heinrichs, R., Soares Filho, C. V. Yield, Yield Components and Nutrients Uptake in Zuri Guinea Grass Inoculated with Plant Growth-promoting Bacteria. International Journal for Innovation Education and Research, v. 8, n. 4, p. 103-124, 2020. DOI: https://doi.org/10.31686/ijier.vol8.iss4.2268

Sá, G. C. R., Carvalho, C. L. M., Moreira, A., Hungria, M., Nogueira, M. A., Heinrichs, R., Soares Filho, C. V. Biomass Yield, Nitrogen Accumulation and Nutritive Value of Mavuno Grass Inoculated with Plant Growth-promoting Bacteria. Communications in Soil Science and Plant ANALYSIS, v. 50, n. 15, p. 1931-1942, 2019(a). DOI: https://doi.org/10.1080/00103624.2019.1648498

Heinrichs, R., Meirelles, G. C., De Melo Santos, L. F., Lira, M. V. da S., Lapaz, A. de M., Nogueira, M. A., bonini, C. S. B., Soares Filho, C. V., Moreira, A. Azospirillum inoculation of 'Marandu' palisade grass seeds: effects on forage production and nutritional status. Semina: Ciências Agrárias, v. 41, N. 2, p. 465-478, 2020. DOI: https://doi.org/10.5433/1679-0359.2020v41n2p465

Carvalho, C. L. M., Duarte, A. N. M., Hungria, M., Nogueira, M. A., Moreira, A., Soares Filho, C. V. Nitrogen in Shoots, Number of Tillers, Biomass Yield and Nutritive Value of Zuri Guinea Grass Inoculated with Plant-Growth Promoting Bacteria. International Journal for Innovation Education and Research, v. 8, n. 5, p. 437-463, 2020. DOI: https://doi.org/10.31686/ijier.vol8.iss5.2360

Leite, R. D. C., Santos, J. G. dos, Silva, E. L., Alves, C. R., Hungria, M., Leite, R. D. C., Santos, A. C. dos. Productivity increase, reduction of nitrogen fertiliser use and drought-stress mitigation by inoculation of Marandu grass (Urochloa brizantha) with A. brasilense. Crop and Pasture Science, v. 70, n. 1, p. 61-67, 2018. DOI: https://doi.org/10.1071/CP18105

Smith, C., Hill, A. K., Torrente-Murciano, L. Current and future role of Haber–Bosch ammonia in a carbon-free energy landscape. Energy & Environmental Science, v. 13, n. 2, p. 331-344, 2020. DOI: https://doi.org/10.1039/C9EE02873K

Hungria, M. Inoculação com Azospirillum brasilense: inovação em rendimento a baixo custo. Londrina: Embrapa Soja. 2011. 36 p. (Documentos, 325).

Garcia, M. M., Pereira, L. C., Braccini, A. L., Angelotti, P., Suzukawa, A. K., Marteli, D. C., Felber, P. H., Bianchessi, P. A., Dametto, I. B. Effects of Azospirillum brasilense on growth and yield compounds of maize grown at nitrogen limiting conditions. Revista de Ciências Agrárias, v. 40, n. 2, p. 353-362, 2017. DOI: https://doi.org/10.19084/RCA16101

Fukami, J., Nogueira, M. A., Araujo, R. S., Hungria, M. Accessing inoculation methods of maize and wheat with Azospirillum brasilense. Amb Express, v. 6, n. 1, p. 3, 2016. DOI: https://doi.org/10.1186/s13568-015-0171-y

Santana, S. R. A., Voltolini, T. V., Antunes, G. dos R., da Silva, V. M., Simões, W. L., Morgante, C. V., Freitas, A. D. S., Chaves, A. R. M., Aidar, S. T., Fernandes-Júnior, P. I. Inoculation of plant growth-promoting bacteria attenuates the negative effects of drought on sorghum. Archives of Microbiology, v. 8, p. 1-10, 2020.

Hungria, M., Campo, R. J., Souza, E. M., Pedrosa, F. O. Inoculation with selected strains of A. brasilense and A. lipoferum improves yields of maize and wheat in Brazil. Plant and soil, v. 331, N. 1-2, p. 413-425, 2010. DOI: https://doi.org/10.1007/s11104-009-0262-0

Rosa, P. A. L., Mortinho, E. S., Jalal, A., Galindo, F. S., Buzetti, S., Fernandes, G. C., Barco Neto, M., Pavinato, P. S., Teixeira Filho, M. C. M. Inoculation with growth-promoting bacteria associated with the reduction of phosphate fertilization in sugarcane. Frontiers in Environmental Science, v. 8, p. 32, 2020. DOI: https://doi.org/10.3389/fenvs.2020.00032

Hungria, M., Nogueira, M. A., & Araujo, R. S. Soybean seed co-inoculation with Bradyrhizobium spp. and Azospirillum brasilense: a new biotechnological tool to improve yield and sustainability. American Journal of Plant Sciences, v. 6, p. 811–817, 2015. DOI: https://doi.org/10.4236/ajps.2015.66087

Hungria, M., Nogueira, M.A., Araujo, R.S. Co-inoculation of soybeans and common beans with rhizobia and azospirilla: strategies to improve sustainability. Biology Fertility Soils, v. 49, p. 791–801, 2013. DOI: https://doi.org/10.1007/s00374-012-0771-5

Duarte, C. F. D., Cecato, U., Hungria, M., Fernandes, H. J., Biserra, T. T., Galbeiro, S., Toniato, A. K. B., Silva, D. R. Morphogenetic and structural characteristics of Urochloa species under inoculation with plant growth-promoting bacteria and nitrogen fertilisation. Crop and Pasture Science, v. 71, n. 1, p. 82-89, 2020. DOI: https://doi.org/10.1071/CP18455

Hanisch, A. L., Balbinot Júnior, A. A., Vogt, G. A. Desempenho produtivo de Urochloa brizantha cv. Marandu em função da inoculação com Azospirillum e doses de nitrogênio. Revista Agroambiente, v. 11, n. 3, p. 200-208, 2017. DOI: https://doi.org/10.18227/1982-8470ragro.v11i3.3916

Fukami, J., De La Osa, C., Ollero, F. J., Megías, M., Hungria, M. Co-inoculation of maize with A. brasilense and R. tropici as a strategy to mitigate salinity stress. Functional Plant Biology, v. 45, n. 3, p. 328-339, 2017. DOI: https://doi.org/10.1071/FP17167

Hungria, M., Nogueira, M.A., Araújo, R.S. Inoculation of Brachiaria spp. with the plant growth-promoting bacterium Azospirillum brasilense: an environment-friendly component in the reclamation of degraded pastures in the tropics. Agriculture, Ecosystems and Environment, v. 221, p. 125–131, 2016. DOI: https://doi.org/10.1016/j.agee.2016.01.024

Silva, H. M. S. D, Vendramini, J. M., Oliveira, F. C. L. de, Soares Filho, C. V., Kaneko, M., Silveira, M. L., Sanchez, J. M. D., Yarborough, J. K. Harvest frequency effects on herbage characteristics of ‘Mavuno’ brachiariagrass. Crop Science, v. 60, N. 2, p. 1113-1122, 2020(b). DOI: https://doi.org/10.1002/csc2.20046

Alvares, C.A., Stape, J.L., Sentelhas, P.C., Gonçalves, J. L. M., Sparovek, K. G. Köppen's climate classification map for Brazil. Meteorologische Zeintschrift, v. 22, p. 711-728, 2013. DOI: https://doi.org/10.1127/0941-2948/2013/0507

CIIAGRO, Centro Integrado de Informações Agrometeorológicas. Rede Meteorológica Automática. 2020. Available in http://www.ciiagro.sp.gov.br/ciiagroonline/Quadros/QTmaxPeriodo.asp. Acessed on august 24th. 2020.

Santos, H. G. dos, Jacomine, P. K. T., Anjos, L. H. C. dos; Oliveira, V. A. de, Lumbreras, J. F., Coelho, M. R., Almeida, J. A., Araujo Filho, J. C., Oliveira, J. B., Cunha, T. J. F. Sistema Brasileiro de Classificação de Solos. 4. ed. Brasília, DF: Embrapa, 2018. 356 p.

Raij, B. van, Andrade, J.C., Cantarella, H., Quaggio, J.A. (eds.) Análise química para avaliação da fertilidade de solos tropicais. Campinas: Instituto Agronômico, 2001. 284p.

Döbereiner, J., Marriel, I., Nery, M. Ecological distribution of Spirillum lipoferum. Canadian Journal Microbiology, v. 22, p. 1464–1473, 1976. DOI: https://doi.org/10.1139/m76-217

Hungria, M.; Araujo, R.S. Manual de métodos empregados em estudos de microbiologia agrícola. Brasília, DF: EMBRAPA - Serviço de Produção e Informação, 1994. p. 542.

Quaggio, J.A., Raij, B. van, Malavolta, E. Alternative use of the SMP-buffer solution to determine lime requirement of soils. Communications in Soil Science and Plant Analysis, v. 16, n. 3, p. 245-260, 1985. DOI: https://doi.org/10.1080/00103628509367600

Silva, D. J., Queiroz, A. C. Análise de alimentos: métodos químicos e biológicos. 3.ed. Viçosa, MG: Universidade Federal de Viçosa, 2002. 235p.

Van Soest, P. J., Robertson, J. B., Lewis, B. A. Methods for dietary fiber, neutral detergent fiber, and non starch polysaccharides in relation to animal nutrition. Journal Dairy Science, v. 74, p. 3583-3597, 1991. DOI: https://doi.org/10.3168/jds.S0022-0302(91)78551-2

Tilley, J. M. A., Erry, R. A. A two-stage technique for the in vitro digestion of forage crops. Grass and Forage Science, v. 18, p. 104–111, 1963. https://doi.org/10.1111/j.1365-2494.1963.tb00335.x. Acesso em 16 ago. 2020. DOI: https://doi.org/10.1111/j.1365-2494.1963.tb00335.x

Van Soest, P. J., Wine, R. H., Moore, L. A. Estimation of the true digestibility of forages by the in vitro digestion of cell walls. Estimation of the true digestibility of forages by the in vitro digestion of cell walls. 1966.

ANKOM. 2017. Neutral detergent fiber in feeds – Filter bags technique (for A200 and A2001). ANKOM Technology. Available in

http://www.ankom.com/09_procedures/procedures6.shtml/. Acessed on august 16th. 2020.

AOAC - Association of Official Analytical Chemists. 1995. Official methods of analysis. 16th ed. AOAC International, Arlington.

Ferreira, D.F. Sisvar: a computer statistical analysis system. Ciência e Agrotecnologia, v. 35, p. 1039-1042, 2011. DOI: https://doi.org/10.1590/S1413-70542011000600001

Rocha, A. F. S., Costa, R. R. G. F. Desempenho de Urochloa brizantha cv. Paiaguás inoculada com Azospirillum brasilense e diferentes doses nitrogênio. Global Science and Technology, v. 11, n. 3, 2018. DOI: https://doi.org/10.18227/1982-8470ragro.v11i3.3916

Sá, G. C. R., Hungria, M., Carvalho, C. L. M., Moreira, A., Nogueira, M., Heinrichs, R., Soares Filho, C. V. Nutrients Uptake in Shoots and Biomass Yields and Roots and Nutritive Value of Zuri Guinea Grass Inoculated with Plant Growth-promoting Bacteria. Communications in Soil Science and Plant Analysis, v. 50, n. 22, p. 2927-2940, 2019(b). DOI: https://doi.org/10.1080/00103624.2019.1689256

Leite, R. D. C., Santos, A. C. D., Santos, J. G. D. D., Leite, R. D. C., Oliveira, L. B. T. D., Hungria, M. Mitigation of Mombasa grass (Megathyrsus maximus) dependence on nitrogen fertilization as a function of inoculation with A. brasilense. Revista Brasileira de Ciência do Solo, v. 43, 2019. DOI: https://doi.org/10.1590/18069657rbcs20180234

Toniato, A. K., Cecato, U., Duarte, C.F.D., Hungria, M., Fernandes, H. J., Biserra, T. T., Sella, M. A. Z., Barreiros, A. R. D. Valor nutritivo do capim-Paiaguás (Urochloa brizantha cv. BRS Paiaguás) inoculado com bactérias promotoras do crescimento vegetal e doses de nitrogênio. In: Embrapa Soja-Artigo em anais de congresso (ALICE). In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE ZOOTECNIA, 55.; CONGRESSO BRASILEIRO DE ZOOTECNIA, 28., 2018, Goiânia. Construindo saberes, formando pessoas e transformando a produção animal: anais... Goiânia: Sociedade Brasileira de Zootecnia, 2018. trab. 0873., 2018.

Salman, A. K., Ferreira, A. C. D., Soares, J. P. G., de Souza, J. P. Metodologias para avaliação de alimentos para ruminantes domésticos. Embrapa Rondônia-Documentos (INFOTECA-E), 2010. 21 p. (Documentos, 136).