Presentation of the levels of Gene Expression of Titanium Dioxide Nanoparticles in Caco-2/HT29-MTX, SAE, and THP-1 cells, using Bioinformatics tools (in silico)

Authors

DOI:

https://doi.org/10.31686/ijier.vol10.iss7.3744

Keywords:

Bioinformatics, Metallic nanoparticles, Nanotoxicology, Database

Abstract

The evolution of studies and learning about the functioning of the human genome made gene expression techniques increasingly explored and applied in recent years' research. There is a need for a deeper understanding of these materials' toxicology in bioinformatics and nanotechnology. Therefore, the present work aims to present the gene expression levels of three studies using three different human cell lines (co-culture of Caco-2/HT29-MTX, SAE, and THP-1 cells), submitted to NPs of carbon dioxide of titanium, via free bioinformatics tools, such as the Limma package (Bioconductor) and Reactome. The data were prepared and standardized by executing the Limma package (Bioconductor), and the results of the most and least expressed genes were used in Reactome. Results show the activation of some genes in common to the experiments, highlighting the co-culture samples of Caco-2/HT29-MTX and THP-1 cells treated with titanium dioxide Nps.

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Author Biographies

  • Josiane Fontoura dos Anjos, Franciscan University and Farroupilha Federal Institute - Alegrete Campus

    Postgraduate Program in Nanosciences – Franciscan University – UFN, Santa Maria, RS, Brazil

    Farroupilha Federal Institute - Alegrete Campus, RS, Brazil
  • Heleno Carmo Borges Cabral, Farroupilha Federal Institute - Alegrete Campus
    Farroupilha Federal Institute - Alegrete Campus, RS, Brazil.
  • Michele Rorato Sagrillo, Universidade Franciscana

    Postgraduate Program in Nanosciences – Franciscan University – UFN, Santa Maria, RS, Brazil

  • Éder Maiquel Simão, Franciscan University

    Postgraduate Program in Nanosciences – Franciscan University – UFN, Santa Maria, RS, Brazil

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Published

2022-07-01

How to Cite

dos Anjos, J. F. ., Carmo Borges Cabral, H., Sagrillo, M. R., & Simão, Éder M. . (2022). Presentation of the levels of Gene Expression of Titanium Dioxide Nanoparticles in Caco-2/HT29-MTX, SAE, and THP-1 cells, using Bioinformatics tools (in silico). International Journal for Innovation Education and Research, 10(7), 39-52. https://doi.org/10.31686/ijier.vol10.iss7.3744